Address
Shristi Enterprises, B-6, Garchaz Complex, Raipur, Chhattisgarh, India
Cat No.
700010005 : 4x20mL/2x10mL
700030005 : 6x40mL/2x30mL
Principle
ALAT
L-Alanine + 2- OxoglutarateL-Glutamate + Pyruvate
LDH
Pyruvate + NADH + H*L— Lactate + NAD*
The rate of NADH consumption is measured photometrically and is directly proportional to the ALAT concentration in the sample.
Reagents: |
||
700010005 |
||
Reagent 1 |
Enzyme solution |
4 x 20 mL |
Reagent 2 |
Substrate solution |
2 x 10 mL |
700030005 |
||
Reagent 1 |
Enzyme solution |
6 x40 mL |
Reagent 2 |
Substrate solution |
2 x 30 mL |
The reagents are stable till the date of expiry, when stored at 2°- 8°C, if contamination is avoided. Do not freeze the reagents.
Mix, 4 parts of reagent 1 and 1 part of reagent 2 = working reagent. The stability of the working reagent is
5 days at 15° -25°C
7 days at 2° - 8°C.
Protect the reaction solution from light.
Reagent Composition
The test has been developed to determine ALAT/GPT concentration within a measuring range from 4 to 700U/L. If such value is exceeded the sample should be diluted 1 + 9 with NaCI solution (9 g/L) and results multiplied by 10.
The lower limit of detection is4 U/L.
Linearity
The linearity is 700 U/L.
Intra-assay precision n = 20 |
Mean (U/L) |
SD (U/L) |
CV (%) |
Sample 1 |
21.9 |
0.72 |
3.28 |
Sample 2 |
35.2 |
0.64 |
0.81 |
Sample 3 |
130 |
0.65 |
0.50 |
Inter-assay precision n = 20 |
Mean (U/L) |
SD (U/L) |
CV (%) |
Sample 1 |
32.5 |
0.37 |
1.13 |
Sample 2 |
37.0 |
0.21 |
0.57 |
Sample 3 |
134 |
0.66 |
0.49 |
Interferences
No interference was observed by Ascorbic acidup to 30 mg/dL, Bilirubinup to 40 mg/dL,
Hemoglobinup to 400 mg/dL,
Triglyceridesup to 2000 mg/dL .
Reagent 1TRIS Buffer
L—Alanine
pH 7.1512.1 g/L
24.1 g/L
LDH (lactate dehydrogenase) Reagent 22-oxoglutarate
NADH
Serum, heparin-plasma or EDTA- plasma
* 1200 U/L
8.5 g/L
1.1 g/L
It is best to measure enzyme activity within few hours of sample collection. There is < 10 % loss of activity within 3 days at 2° - 8°C and <17 % at 15° -25°C.
Assay Procedure Wavelength340 nm
Light path10 mm
Temperature37°C
Sample |
100 pL |
Working reagent |
1000 pL |
Mix, incubate for 1 min. andread absorbance afterevery 1 min.for 3 min. |
ALAT activity [U/L] =AA/min x factor
For internal quality control DiaSys TruLab N and P controls should be assayed with each batch of samples.
For the calibration of automated photometric systems the DiaSys TruCal U calibrator is recommended.
Reference Range
Women |
< 31 U/L |
Men |
< 41 U/L |
“Each laboratory should check if reference ranges are transferable to its own patient population and determine own reference ranges if necessary”.
not swallow. Avoid contact with skin and mucous membranes.
Quick References :
Parameter |
ALAT/ GPT |
Mode |
Kinetic |
Reaction slope |
Decreasing |
Wavelength |
340 nm |
Path length |
10 mm |
Temperature |
37°C |
Working reagent |
1000 pL |
Sample volume |
100 pL |
Delay |
60 sec. |
Rate |
60 sec. |
No. of readings |
3 |
Factor |
1745 |
Normal range |
Male : < 41 U/L |
Female: < 31 U/L |
|
Linearity |
700 U/L |
"Providing quality product with right information of latest technology to cater requirement of medical professionals."
Shristi Enterprises has been providing excellent services for last many years. The support services provided are very good and they are very prompt in dealing with any of the issues pertaining to instruments.
Shristi Enterprises is dedicated for health care services always ready to provide prompt services to the customer whenever needed . Executives and dealer are ready to help any time .
Amit Sharma
Founder
Hi, I am Amit Sharma, founder of Shristigroup. I started this organization in 2001. Since inception my vision is to make Shristi Enterprises the number one organization in medical & healthcare supplies in Chattishgarh, INDIA, After achieving the same, now I am looking to expand its operation across India.
We have a team of 50+ workforce which include people who have worked at top positions in Sales & Customer Service of reputed medical instrument manufacturer companies. We have the state of the art Infrastructure which includes 10,000 sqft warehouse in Raipur (our HQ) & satellite offices in Bhopal & Indore (M.P.) & in Mumbai. Apart from warehousing, we have our own transportation system to ensure timely & safe deliveries to all our customers.
For product sustainability, material storage and logistics is number one priority for us. We have our in-house supply chain management integrated with freight forwarding and contract logistics to provide seamless distribution process from manufacturers to our customers.
We also use latest softwares to ensure we operate at maximum efficiency. We have state of the art, third party developed accounting, employee management, inventory management & delivery tracking software.
Our satisfied customers include AIIMS Raipur, Bhilai Steel Plant, Government Medical College Raipur and various private hospitals in MP, CG and rest of INDIA.